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KMID : 1001020100080010027
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Journal of Urologic Oncology
2010 Volume.8 No. 1 p.27 ~ p.31
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Evaluation of Total RNA Extraction Methods for OCT Compound -Embedded Frozen Prostate Tissue Sections
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Jeon Sung-Yoon
Lee Hyoung-Nam
Kim Tae-Jung
Jung Eun-Sun
Lee Kyo-Young
Choi Yeong-Jin
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Abstract
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Purpose: There are two methods commonly used for the extraction of total RNA. One is a Trizol method and the other is a column method. However, there have been few reports comparing the quality of total RNA extracted by these two methods in fresh frozen OCT compound-embedded (FFOE) tissues. We evaluate the two RNA extraction methods, to know the best quality of total RNA in FFOE tissues.
Materials and Methods : Twenty- one fresh frozen human prostate tissues were used for RNA extraction by the classic method using Trizol reagent and the commercially available method, respectively. RNA purity and quality analysis were performed by spectrophotometry and automated electrophoresis system.
Results: An A260：A280 ratio of all RNA from two methods were above of 1.8. The ratio of 28S/18S rRNA in Trizol method (tumor=0.93±0.25, nontumor=1.03±0.28) were higher than the column method (tumor=0.58±0.20, nontumor= 0.60±0.28, p＜0.001). On gel images, 18S and 28S rRNA were separated and intact in Trizol method but they were not visible as intact bands but fragmented in column method.
Conclusions : To purify the best quality RNA from the FFOE tissues for the molecular pathologic study, Trizol method is superior to the column method.
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KEYWORD
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RNA, Frozen sections, Prostate, Method
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